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For example, if an unknown culture grows on MacConkey Agar, it is most likely a Gram negative species. One can then test it on TSI to test for its hydrogen sulfide production and utilization of sugar both aerobically and anaerobically to quickly narrow its possible identity. One drawback of Combination Differential Media is that their test results may be quite complex because there are usually many different possible results from the multiple tests being run at the same time.

Color-Removal by Microorganisms Isolated from Human Hands

In addition, sometimes one test result may obscure another test result, but usually an experienced microbiologist can detect the obscuration. For example, with the TSI test, sometimes the production of hydrogen sulfide makes the tube so black that one is unable to see a yellow color from the anaerobic utilization of sugar.

Journal of Agronomy.

Year: Volume: 13 Issue: 3 Page No. Abstract: Endophytic bacteria is bacteria living in plant tissue and can be isolated through sterilization of tissue surface. Exploration of endophytic bacteria from rubber plant, that are potentially involved in enhanching growth, is important to be carried out.

Culture Media in Microbiology

The objective of this experiment was to characterize and select the endophytic bacteria from rubber plants that had potency to enhance growth. Endophytic bacteria were isolated from sample taken from leaf, shaved bark and feeder root of IRR and IRR 39 rubber clones. The endophytic bacteria were found from isolation and then they were selected by hypersensitive response and hemolysis test and through germinating and growth test, the best five endophytic bacteria were selected.

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